Other Interdisciplinary Fields
Confocal microscopy uses a laser light source to excite fluorescent probes within cells, thereby generating fluorescence images of subcellular fine structures inside cells or tissues. Consequently, high‑resolution imaging can be performed on a wide range of samples labeled with fluorophores. Consequently, high‑resolution imaging can be performed on a wide range of samples labeled with fluorophores.
Building on the principles of wide-field microscopy, the laser scanning confocal microscope uses a laser beam as its light source. The laser beam passes through an illumination pinhole, is reflected by a beamsplitter to the objective lens, and is focused onto the sample, where it scans the focal plane point by point. If the sample contains fluorophores that can be excited, the emitted fluorescence travels back along the original excitation path, re‑entering the beamsplitter. After passing through a detection pinhole—where the light is further focused—the fluorescence is detected by a photodetector, and the resulting signal is transmitted to a computer, which reconstructs a fluorescent image. In the optical design, only light from the focal plane is allowed to pass through the detection pinhole; light originating from regions outside the focal plane is blocked. Consequently, the background at non‑focused locations appears dark.
Case study
Starch is an organic compound produced by green plants through photosynthesis and serves as a long-term energy reserve. It is a graded, widely utilized polysaccharide found in small granules within the stems, roots, grains, and fruits of green plants. Studies have shown that the growth rings of starch granules gradually disappear as temperature increases. The MEAGLE 100 instrument enables high‑resolution imaging of these growth rings, allowing for quantitative analysis of how their morphology evolves with changing temperatures.
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