Pathology
A medical process that helps investigate the etiology, pathogenesis, pathological changes, outcomes, and prognosis of diseases. Pathological research methods can involve quantitative analysis based on cellular size, shape, and color, as well as nuclear size, shape, and number; they can also leverage antigen–antibody specific binding to detect unknown antigens or antibodies in tissues, with practical applications spanning etiological diagnosis, immunological disease diagnosis, and tumor diagnosis. In such studies, the MEAGLE 100 employs immunofluorescence to quantitatively observe and monitor antigen expression, cellular structural features, and the effects and mechanisms of anti‑tumor drugs in tumor cells. Coupled with its built-in cell‑analysis capabilities, it enables simultaneous imaging and quantitative assessment of cellular and subcellular morphology, quantity, intensity, and other parameters.
Case study
Tumor research typically begins by labeling specific antigens or antibodies with fluorescent dyes, allowing them to bind to their corresponding antibodies or antigens. The samples are then imaged using the MEAGLE 100 confocal microscope for qualitative and quantitative analyses as well as morphological characterization. For instance, the localization, identification, and quantification of tumor-associated antigens such as P53 can be achieved by fluorescently labeling certain protein molecules and subsequently measuring their mean fluorescence intensity and integrated fluorescence intensity, thereby enabling quantitative analysis of specific cellular structural proteins.
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