Laser Confocal Microscope
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Release time：2022-10-04
Summary： Our company’s laser confocal microscope uses a laser beam as its light source. The laser beam passes through an illumination pinhole, is reflected by a beamsplitter to the objective lens, and is focused onto the sample, enabling scanning of every point in the specimen’s focal plane.
I. Introduction
Our company’s laser confocal microscope uses a laser beam as the light source. The laser beam passes through an illumination pinhole, is reflected by a beamsplitter to the objective lens, and is focused onto the sample, enabling scanning of every point in the specimen’s focal plane. Fluorescent molecules within the tissue sample, upon excitation, emit fluorescence that travels back along the original illumination path to the beamsplitter. After passing through a detection pinhole for further focusing, the focused light is detected and collected by a photomultiplier tube (PMT), with the resulting signal transmitted to a computer. The computer then reconstructs the image and displays it on a monitor.
II. Applications
Our company’s laser confocal microscopes enable the observation of fixed cells and tissue sections, as well as real-time, dynamic imaging and analysis of cellular structures, molecules, and ions in living cells. They are widely employed in studies of cellular morphological localization, three-dimensional structural reconstruction, and dynamic processes, and provide robust tools for quantitative fluorescence measurements and quantitative image analysis. When integrated with other relevant biotechnologies, these instruments have also found extensive applications in molecular and cellular biology fields such as morphology, physiology, immunology, and genetics.
III. Significance
Our company’s laser confocal microscope can analyze certain physical and biochemical properties of cells, as well as monitor antigen expression, cell–cell interactions, and cytotoxic activity. In brain biopsy specimens from patients with multiple sclerosis, we observed that microvascular endothelial cells within the lesion tissue exhibit specific expression patterns. This technology helps biomedical researchers harness science to advance human health.
Keywords:
Microscope,Laser beam
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